The Role of trnH-pbsA Spacer Gene in Eucalyptus Species Identification and its Importance in Phylogenetics

Abstract

Our present study is made to use the trnH-psbA spacer gene data to resolve some phylogenetic relationships among the Eucalyptus speices. The species included are E. moorei, E.affn. moorei, E. dives, E. mitchelliana, E. pauciflora and E. stellulata. There were successful amplifications of DNA for the seven Eucalyptus species using gene trnH-psbA. The trnH-psbA spacer is the short with 476 bps. It is to be complimented with rbcL, MatK and trnH-psbA gene study. The trnH-psbA spacer is showing 21 SNPs out of 476 bps segment. The mean length is 733 bps long. Amplification success rate is almost 100%. For each species there are multiple good quality sequences available for the sequence alignment. The trnH-psbA spacer has discriminated 5 species clearly from the rest and the other 3 species are also separated from each other though the distance between them is not significantly noticeable.