Unveiling the Effects of Cisplatin and Diallyl Disulfide on MDA-MB-231 Breast Cancer Cells

Authors

  • Kaavya Gunasekaran Department of Biochemistry, Bharathiar University, Coimbatore – 641 046, India.
  • Priyadharshini Thangavelu Department of Biochemistry, Bharathiar University, Coimbatore – 641 046, India
  • Priyadharshini Thangavelu Department of Biochemistry, Bharathiar University, Coimbatore – 641 046, India
  • Naveen Kumar Kalagatur DRDO-BU-Center for Life Sciences, Coimbatore – 641 046, India.
  • Rama Jeyaraj Jindal Institute of Behavioral Sciences (JIBS), Jindal Global Institution of Eminence Deemed to Be University, Sonipat – 131 001, India, Director of Clinical Sciences, Northern Territory Institute of Research and Training, Darwin, NT 0909, Australia
  • Suja Samiappan Department of Biochemistry, Bharathiar University, Coimbatore – 641 046, India.

DOI:

https://doi.org/10.5530/ctbp.2024.3.29

Keywords:

Cancer, wound healing, colony formation, apoptosis, cell cycle, Haemolysis

Abstract

Cancer is one of the most aggressive diseases and is the primary cause of mortality around the world. This can be prevented by combining anti-cancer drugs to reduce the resistance to monotherapy and thereby reduce the toxic effects. Cytotoxic anticancer drugs can potentially elicit cancer cell death by apoptosis or necrosis. Our present study aimed to investigate the combined cytotoxic potential of cisplatin (CDDP) and diallyl disulfide (DADS) on MDAMB-231 breast cancer cell lines. The clonogenic assay was also performed to assess the effects of the drug on the proliferation of breast cancer cells. The results showed that CDDP/DADS (CDDP and DADS) markedly inhibited cell proliferation and significantly reduced the colony formation potential, migration, and invasion abilities of MDA-MB-231 cells. The apoptosis assay confirmed that cell death was through an apoptotic pathway. Cell cycle analysis results indicated that the combination effect of the drugs resulted in arresting cells in the G2/M phase of the cell cycle. Further, the haemolytic assay revealed that the CDDP/DADS is nontoxic to RBCs. In conclusion, combining these two drugs inhibits the oncogenic properties of TNBC cells, including their growth, survival, migration, and invasiveness.

The percentage of MDA-MB-231 cells  that survived after treatment with CDDP/DADS  compared to the control at 12 and 24 h.

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Published

31-07-2024

How to Cite

Gunasekaran, K. ., Thangavelu, P., Thangavelu, P., Kalagatur, N. K. . ., Jeyaraj, R. ., & Samiappan, S. . (2024). Unveiling the Effects of Cisplatin and Diallyl Disulfide on MDA-MB-231 Breast Cancer Cells. Current Trends in Biotechnology and Pharmacy, 18(3), 1813–1821. https://doi.org/10.5530/ctbp.2024.3.29