Identification of Phytoconstituents and investigation of nephroprotective potential of seeds of Rumex vesicarius in experimental animals

Abstract

The present study is planned for phytochemical analysis and to evaluate the nephroprotective effect of methanol seed extract of  Rumex vesicarius on cisplatin-induced nephrotoxicity. The study initiated with pharmacognostic studies of seeds followed by preparation of methanol extract by using hot extraction method. Then the preliminary phytochemical studies were performed followed by  HPLC and GC-MS analysis. Further in vitro antioxidant studies were performed by adopting DPPH, ABTS and NO techniques. Further Pharmacological studies were initiated with acute toxicity studies. Nephroprotective activity in cisplatin induced model is evaluated by the determination of levels of serum markers and urinary functional parameters. Phytochemical analysis revealed the presence of bioactive phytoconstituents. HPLC revealed in the presence of rutin, whereas GC-MS analysis of methanolic extract resulted in 108 compounds. Upon in vitro antioxidant studies the seed extract exhibited markable antioxidant activity. Animal studies showed that methanol extract successfully ameliorated the nephrotoxicity induced by cisplatin in dose dependent passion.  Hereby the current study resulted in identification of phytochemical compounds in seeds of Rumex vesicarius and also scientifically validated the ethnomedicinal use of seeds of Rumex vesicarius in attenuation of renal problems.