Protective Effect of Annona Squamosa Fruit Pulp on Motor Responses Following Intra-Medial Forebrain Bundle Injection of 6-Ohda In Rat Model of Parkinson Disease

Abstract

Objective:The present study investigated the ethanolic extract of fruit pulp of Annona squa-mosa that ameliorates the 6-OHDAinduced behavioural, biochemical, and neurochemical changes which resemble Parkinson’s disease (PD)-like symptoms. Materials and Methods:Various behavioural and biochemical parameters were carried out to evaluate the activity of ethanolic extract of fruit pulp of Annona squamosa (FEAS) on 6-OHDA treated rats. To determine the therapeutic sig-nificance of FEAS on PD, different behaviour-al tests such as apomorphine rotation, narrow beam maze, rotarod, grip strength, sensorimo-tor and disengage test and some biochemical tests along with neurochemical findings were done.Results:6-OHDA caused physical and behavioral abnormalities in animals, includ-ing abnormal posture, weak grip strength, and motor deficit. Biochemical analysis of brain ho-mogenates in FEAS treated rats showed altered oxidative stress and elevated lipid biomarkers. Neurochemical alterations of the striatum of FEAS treated rats exhibit altered levels of cat-echolamines. FEAS administered for 07 days significantly enhanced motor function and be-haviour tasks and further restored the invitro antioxidant changes in the brain. Furthermore, FEAS-II treatment significantly improved oxida-tive damage,which is denoted by the alterations in neurochemical changes of rat brain.Conclu-sion:In this research work, FEAS-I & II (200 mg/kg & 400 mg/kg) provided a remarkable neuro-protective impact, which was evidenced by be-havioural and biochemical tests. It restored the behavioural and biochemical alterations caused by 6-OHDA and confirmed the strong neuropro-tective mechanism of FEAS in 6-OHDA-intoxi-cated behavior and motor abnormalities