Antihyperglycemic Effect of Annona squamosa Leaf and Oleanolic Acid Combination in Diabetic Albino Rats

Abstract

<p style="text-align: justify;">Since form ancient time, plant derived product has been used as holistic approach in the control of diabetes mellitus. The object of the study is evaluation of the synergistic anti-hyperglycemic effect of medicinal plant <em>Annona squamosa</em> leaf extract (ASLE) and plant-derived oleanolic acid (OA) in streptozotocin (STZ)-in-duced diabetic rats. Thirty six number of Wistar rats are taken and divided within six groups (<em>n</em> = 6). The group 1 is normal control, group 2 is diabetic control induced with STZ, groups 3, 4, 5, and 6 treated with 50 mg/kg of ASLE, 50 mg/kg of OA, the combination of both 25 mg/kg of ASLE and 25 mg/kg of OA, and 0.1 mg/kg of glimepiride respectively for 28 days. The pa-rameters like body weight, blood glucose, insu-lin, glycosylated hemoglobin, C-peptide, serum biochemical, lipid profiles and liver antioxidant parameters were evaluated. Results shows sig-nificant (<em>p</em> &lt; 0.05) restoration of body weight, insulin and C-peptide levels. The animal groups treated with the combination of both ALSE and OA have experienced a remarkable restoration of serum biochemical, lipid profiles, antioxidant parameters, and C-peptide level as compared to the above groups due to the synergistic ef-fect. These findings conclude that combination of both ASLE and OA had a synergistic action and it shows more potent anti-hyperglycemic ef-fect.</p>