Study of the Genetic Variations in Different Variants of Tribulus terrestris L. in Rayalaseema Region in Andhra Pradesh

Abstract

Tribulus terrestris (Family - Zygophyllaceae) is a finely known medicinal herb, usually called as palleru in Andhra Pradesh. This is seen all over the world along with India and is used in diverse systems of medicine such as diuresis, chronic cystis, calculous affection, incontinence of urine, impotency, gonorrhoea, gleet, spermetorrhoea, and phosphateurea. Tribulus terrestris is one of the components of Dashamoola, is an ancient Ayurvedic formulation made up of ten medicinal herbs which is used in various Ayurvedic medicines and as well as in chyavanprash. And used as tonic in Unani medicine. In the present study 8 different regions of Tribulus terrestris plant leaves were collected which is further subjected to molecular characterization by RAPD analysis. In present days RAPD is used for trace the phylogeny in both animal and plant diverse. RAPD analysis found that various mild variations with respect to different regions of Kurnool district, Rayalaseema region of Andhra Pradesh. It was observed that all 15 primers were found to generate clear and polymorphic bands. Total number of bands produced was 223 in which 159 shows polymorphic bands. The percentage of polymorphism obtained by 15 primers was 71.3%.