In Vivo Cardioprotective Effect of With a coagulin Isolated from Withania coagulans Fruits on Isoprenaline- Induced Myocardial Injury in Experimental Rats

Abstract

Myocardial infarction (MI) (ie, heart attack) is the irreversible death (necrosis) of heart muscles. The possible cardioprotective effect of withacoagulin isolated from the fruits of Withania coagulans against isoprenaline-induced myocardial injury has been investigated in rat model in this study.

Albino rats (120 ± 10 g) were divided into five groups (n=6) as follows: Group (1) control, Group (II) Toxic control (ISO 85 mg/kg, s.c.), Group (III) per se (Withacoagulin 25 mg/kg, p.o.), Group (IV) Treatment 1 (WC+ ISO) and Group (V) Treatment 2 (Vit E+ ISO). To assess the efficacy of WC treatment against ISO-induced cardiotoxicity, lipid peroxidation, biochemical parameters and histopathological examinations were conducted.

Administration of ISO induced severe myocardial injury and altered lipid peroxidation. However, WC pretreatment secured cardiovascular derangements, and could be correlated by amelioration in lipid profiles such as triglyceride, total cholesterol, lactate dehydrogenase, LDL-cholesterol and HDL-cholesterol. In addition to these, cardiac indexes, atherogenic indexes, and coronary artery indexes were significantly improved in Group (IV) when compared to ISO intoxicated animals. Moreover, WC administration to ISO-treated rats obviously mitigated the malondialdehyde level when compared to Group (II). The histopathological observations also demonstrated that WC pretreatment significantly restored the damage induced by ISO.

These results suggests that WC improved myocardial injury and could be recommended as a potential candidate for the development of novel cardioprotective agents.