Global Regulatory Frameworks for Multiplex Genome Editing: A Bibliometric Analysis

Abstract

The demand for efficient and sustainable food production systems has increased due to the world’s growing population and shrinking arable lands. The creation of high-yielding, nutritionally improved, and stress-tolerant cultivars has become crucial in order to meet these challenges. With the introduction of sophisticated genome editing tools, particularly Multiplex Genome Editing (MGE). methods like CRISPR-Cas9, traditional breeding practices and early genetics alteration tactics have changed. These technologies provide promising strategies for enhancing crop resilience against pests, diseases, and environmental stressors by enabling precise and simultaneous changes at numerous genetic loci. This study uses bibliometric analysis to explore the landscape of MGE research in crop improvement using data from Web of Science and Scopus (2016- 2025), tools like VOSviewer and Bibliometrix in R studio are used to generate visualizations of collaboration networks, coauthorship, keywords trends, and citation patterns. The analysis reveals major research domains that are driving progress in the field, such as yield enhancement, trait stacking, metabolic engineering, and gene regulation, as well as leading authors, institutions, and journals that are driving this field. Therefore, a thorough bibliometric analysis of MGE in crop improvement utilizing CRISPR-cas9 over the last ten years is needed. The current efforts attempt to close this research gap by providing academics with multi-dimensional insights into current and future developments in the promising field.