Determination of Antibiotic Residues in Ice Creams by Using LC/MS/MS Technique

Abstract

Two different brands of ice creamhave been procured from the market and used for the present study.Five commonly used antibiotics such as Nitrofuran metabolites,Sulfonamides, quinolines &Fluoroquinolone residue,Nitrofuran Parent compounds, Nitroimidazole, Chloramphenical and tetracycline.0.1 grams of standard were dissolved in 2 milliliters of methanol to create stock solutions. To create a standard working solution with varying concentrations, this solution was further diluted using the same solvent.Samples were analyzed for antibiotic residues using HPLC coupled with triple quadrupole mass spectroscopy (LC/MS/MS). The separation was carried out using isocratic mode on an Agilent ODS C18 column with a suitable mobile phase.The technique's linearity, recovery, accuracy, and specticity were all confirmed.