Simultaneous Quantification of Para-Phenylenediamine and Resorcinol in Hair Dyes Using Franz Diffusion Technique Coupled With HPLC

Abstract

Hair dyes commonly contain paraphenylenediamine (PPD) and resorcinol, which are widely used as oxidative coloring agents. Due to their potential skin penetration and associated health risks, it is crucial to assess their permeation through the skin and quantify their levels in cosmetic formulations. This study aims to determine the diffusion and quantification of PPD and resorcinol using the Franz diffusion cell technique coupled with high-performance liquid chromatography (HPLC). The experimental setup involves applying commercially available hair dye formulations onto a synthetic membrane in a Franz diffusion cell to simulate dermal absorption. Samples from the receptor compartment are collected at predetermined intervals and analysed using an optimized HPLC method. The chromatographic separation is performed on a C18 column with a suitable mobile phase, ensuring efficient resolution and detection of both compounds.Method validation follows ICH guidelines, assessing parameters such as linearity, accuracy,precision, and sensitivity. This study provides critical insights into the skin permeability of PPD and resorcinol, contributing to the safety assessment of hair dye formulations. The developed analytical method ensures precise quantification, supporting regulatory compliance and consumer safety in cosmetic product development.