Essential Metals (Calcium and Znic) and Chelating Agent (Monoisoamyl 2, 3-dimercaptosuccinic acid) Attenuates Arsenic Induced Neurochemical Alterations in Rats

Authors

  • K Praveen Kumar Department of Biotechnology, S.V.University, Tirupati-517502, India
  • S Saritha Department of Biotechnology, S.V.University, Tirupati-517502, India
  • G Rajarami Reddy Department of Zoology, S.V.University, Tirupati-517502, India

DOI:

https://doi.org/10.5530/ctbp.2021.4.37

Keywords:

Arsenic developmental toxicity,, Essential metals, MiADMSA, Brain regions

Abstract

Arsenic (As) is ubiquitous in environment and well known developmental neurotoxicant. In the present study we examined the protective effect of calcium (Ca), zinc (Zn) and monoisoamyldimercaptosuccinic acid (MiADMSA), either individually or in combination against As induced alterations in catecholamine levels, acetylcholine (ACh), acetyl cholinesterase (AChE) and monoamine oxidase (MAO) in brain regions (cerebral cortex, hippocampus and cerebellum) of PND 21, PND 28 and 3 month old rats. Gestational and lactational (GD6 to PND 21) As exposure significantly decreased the levels of catecholamines (dopamine, epinephrine and nor epinephrine), AChE and MAO activities with an increase in ACh content in all selected age points. The observed alterations in these neurochemcical parameters were higher at PND 28. Among the brain regions, As induced alterations in catecholamines levels and cholinergic system were more pronounced in hippocampus than cortex and cerebellum. Individual supplementation of essential metals (Ca or Zn) or chelating agent (MiADMSA) significantly reversed the As induced neurochemical alterations. However, combined supplementation of essential metals with the chelating agent produced greater reversal effects in reducing As induced neurotoxicity.

Effect of arsenic on epinephrine in different brain regions (cerebral cortex (a), cerebellum (b), hippocampus (c)) of PND 21, PND 28 and 3 months old rats and the reversal effects of calcium, zinc or MiADMSA, either individually or in combination. Rats were exposed to either deionized water (control) or As (100 ppm) or supplementation of calcium and zinc (individual 10 ppm each or combined 5 ppm each) together with As from gestation day 6 to PND 21. MiADMSA was given to pups from PND 18 to PND 20, either individually (50 mg/kg b.wt) or in combination (25 mg/kg b.wt) with calcium and zinc to As. Values represent Mean ± S.D (n=6). The 0.05 level of probability was used as the criterion for significance. Mean values in a row that share the same superscript do not differ significantly at P <0.05.

Downloads

Published

2021-12-23

How to Cite

Praveen Kumar, K., Saritha, S., & Rajarami Reddy, G. (2021). Essential Metals (Calcium and Znic) and Chelating Agent (Monoisoamyl 2, 3-dimercaptosuccinic acid) Attenuates Arsenic Induced Neurochemical Alterations in Rats. Current Trends in Biotechnology and Pharmacy, 15(4), 368–376. https://doi.org/10.5530/ctbp.2021.4.37