An interesting approach for Biofilm disruption by Thermocouple device

Authors

  • Krithika Parthiban Department of Biotechnology, Mount Carmel College, Autonomous, Bengaluru, 560001, India
  • Pragya Tamarakar Department of Physics, Mount Carmel College, Autonomous, Bengaluru, 560001, India
  • Mahua Ghosh Department of Microbiology, Muralidhar Girls College, West Bengal- 700029, India
  • Priya Gopal Department of Microbiology, Muralidhar Girls College, West Bengal- 700029, India
  • Prosun Tribedi Department of Biotechnology, Microbial Ecology Laboratory, The Neotia University, West Bengal-743368, India
  • Bannhi Das Department of Biotechnology, Mount Carmel College, Autonomous, Bengaluru, 560001, India

DOI:

https://doi.org/10.5530/ctbp.2021.3s.52

Keywords:

Bacterial biofilm, Thermocouple device, Low voltage electric shock, Pseudomonas sp., Staphylococcus sp

Abstract

Microbial biofilm are an aggregate of microbial population encompassed in a matrix of self secreted extracellular polymeric substances (EPS). In various sectors from human health to industries biofilm bring with it an array of harmful impacts. For instance, biofilm linked diseases like chronic otitis, chronic osteomyelitis, urinary tract infections, cystic fibrosis-associated lung infection etc. cannot be treated with regular antibiotics since profound use of the same have led to the existence of several drug resistant strains. Effective techniques for combating this bacterial biofilm are still a challenging issue to the scientific community. Towards this direction, in the present study efforts have been put to use electric shock generated by a thermocouple device to disrupt bacterial biofilm formation. The uniqueness of the procedure lies with the method of generation of the electric shock, wherein a difference in temperature has been exploited to create the shock. Using this method, electric shocks of 105μV was applied for different durations on bacterial biofilm formed by Pseudomonas sp. and Staphylococcus sp. In each case a difference in response was observed which led to the conclusion that Staphylococcal biofilm as well as Pseudomonal biofilm are susceptible to the electric shocks. Hence, this interesting method of usage of low voltage electric shock could be a potential method to fight against biofilm threats.

Standardisation of Biofilm formation by Pseudomonas sp and Staphylococcus sp. CV assay was performed after 24hr, 48hr and 72hr of inoculation, for biofilm estimation of both Pseudomonas sp. and Staphylococcus sp. Absorbance measured at 580nm.

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Published

2021-11-01

How to Cite

Parthiban, K., Tamarakar, P. ., Ghosh, M. ., Gopal, P. ., Tribedi, P. ., & Das, B. . (2021). An interesting approach for Biofilm disruption by Thermocouple device. Current Trends in Biotechnology and Pharmacy, 15(5), 545–549. https://doi.org/10.5530/ctbp.2021.3s.52