Formulation and Characterization of Transdermal Patch Containing Eucalyptus, Curcumin and Ginger Oils with its In-vitro Permeability Study Using Goat Skin and GC-MS Analysis

Abstract

The present research has been carried out to formulate the transdermal patch containing medicinal oils like eucalyptus, curcumin, and ginger with its characterization and in-vitro permeability checking by GC-MS analysis. Three formulations (F1, F2, and F3) of transdermal patches containing different concentrations of oils were prepared. The patches were evaluated for thickness, weight uniformity, percentage moisture content, and percentage moisture uptake. The average thickness was found to be 0.434, 0.433, and 0.438 mm for F1, F2, and F3 respectively. 0.1876, 0.1861, and 0.1866 gm weight uniformity were observed for F1, F2, and F3 respectively. The moisture content for F1 was 3.96 %, for F2 was 2.48 % and for F3 was 3.67 %. The moisture uptake for F1 was 5.68 %, for F2 was 4.32 % and for F3 was 4.82 %. The F2 formulation produced optimum results, so it is better than F1 and F3. The invitro permeation of oils from the transdermal patch was checked by Franz diffusion cell using goat skin as a semi-permeable membrane. The transferred sample was collected and investigated by chemical identification tests at time intervals of 1, 2, 3, 4, and 5 hours for the presence of active chemical constituents. The GC-MS analysis showed the presence of active chemical moieties which transferred through the goatskin including D-limonene, (+)-4-carene, Fenchone, linalool, endo-borne, citronellal, 2,6-octadienal,3,7-dimethyl-,(Z)-,longifolene, caryophyllene, carotol, diethyl phthalate, etc. The above research proves our approach to formulation and delivery of polyherbal oils in a single transdermal patch which may open the door for delivery of formulation containing herbal oils through the skin.