Isolation and Characterization of Protease Producing Novel Burkholderia sp.PS1 from Soil Sample and its Protease Production Optimization Studies

Abstract

Extracellular proteases, due to their commercial, industrial applications have become significant and targeted for scientific research during current research. Our present report states about unfolding of potential proteases from soil sample. Even though there are diversified protease positive microorganisms was reported but still few are Burkholderia Cenocepacia proteases are unrevealed. Selective soil sample was collected from different places of Mahbubnagar Telangana state for screening and isolated potential protease producing microorganisms. 10-5 diluted sample spread over skim milk agar (High Media) and selected 108 protease positive organisms from screening. selected isolates are pushed to secondary screening, picked highest zone of hydrolysing microorganism was identified through 16s rRNA generated 1358bp amplicon was forwarded to NCBI blast, 98% homology Burkholderia Cenocepacia (Bks) submitted and generated accession number MH290479 with optimized Bks protease production showing maximum production at temperature 30oC, PH -7, Inoculum size 3%, 1% glucose as carbon source,0.5% Gelatine protein and Zn metal ion was recorded.