Enhancing Breast Cancer Treatment: Investigating the Influence of Polymer Ratios on Luteolin-Loaded TPGS/Poloxamer Micelles

Authors

  • Muhammad Redza Fahmi bin Mod Razif University of Cyberjaya, 63000 Cyberjaya, Selangor, Malaysia
  • Kai Bin Liew University of Cyberjaya, 63000 Cyberjaya, Selangor, Malaysia
  • Siok Yee Chan Universiti Sains Malaysia, 11700 Penang, Pulau Pinang, Malaysia
  • Yik Ling Chew Department of Pharmaceutical Chemistry, UCSI University, Kuala Lumpur, Malaysia
  • Masriana Hassan Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
  • Shairyzah Ahmad Hisham University of Cyberjaya, 63000 Cyberjaya, Selangor, Malaysia
  • Shamima Abdul Rahman University of Cyberjaya, 63000 Cyberjaya, Selangor, Malaysia
  • Phei Er Kee Yuan Ze University, 320 Zhongli, Taoyuan, Taiwan

DOI:

https://doi.org/10.5530/ctbp.2024.3s.16

Keywords:

Luteolin, TPGS, Poloxamer, Optimization, Micelle, Breast cancer

Abstract

Luteolin is a widely studied flavonoid recognized for its ability to sensitize multidrug-resistant cells and anti-cancer properties. However, its clinical applications is hindered by challenges arising from its limited solubility and bioavailability. To address this issue, a synergistic approach combiningluteolin with vitamin E TPGS (TPGS) and poloxamer (Pol) was explored to boost tumour apoptosis and suppress P-glycoprotein. This study aimed to optimize luteolin-loaded TPGS/Pol micelles developed using the film hydration method, followed by lyophilization. Various key factors including the drug: polymer ratio and the TPGS: Pol ratio were examined, with encapsulation efficiency (EE) assessed using a UV-Vis spectrophotometer and particle size measured via dynamic light scattering (DLS). The results revealed that the optimized micelle composed of 1:5 drug: polymer ratio and a TPGS: Pol ratio of 3:1, exhibited a particle size below 40 nm, along with EE of 90%. Additionally, there was a notable increase of 459-fold in the solubility of luteolin-loaded micelle in a critical micelle concentration (CMC) comparison to pure luteolin in water. The TPGS/Pol micelles demonstrated a critical micelle concentration presented the feasibility of TPGS/Pol micelles in improving the therapeutic potential of luteolin, showcasing improvements in EE, particle size, solubility and sustained release behaviour.

Particle size obtained by transmission electronic microscope (TEM) (left) and dynamic  : Particle size obtained by transmission electronic microscope (TEM) (left) and dynamic Particle size obtained by transmission electronic microscope (TEM) (left) and dynamic  light scattering (DLS) (right)

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Published

02-10-2024

How to Cite

Muhammad Redza Fahmi bin Mod Razif, Kai Bin Liew, Siok Yee Chan, Yik Ling Chew, Masriana Hassan, Shairyzah Ahmad Hisham, Shamima Abdul Rahman, & Phei Er Kee. (2024). Enhancing Breast Cancer Treatment: Investigating the Influence of Polymer Ratios on Luteolin-Loaded TPGS/Poloxamer Micelles . Current Trends in Biotechnology and Pharmacy, 18(3s), 209–218. https://doi.org/10.5530/ctbp.2024.3s.16