The cytotoxic and anti-depressant activity of ethanol extract of Pterospermum semisaggitatum (buch. ham.exroxb.) leaves: an in vitro and in vivo study

Authors

  • Mohammad Hamid Ullah Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Cyberjaya, Selangor, Malaysia
  • Masro Mohamad Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Cyberjaya, Selangor, Malaysia
  • Shamima Abdul Rahman Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Cyberjaya, Selangor, Malaysia
  • Mohammed Abu Sayeed Department of Pharmacy, International Islamic University Chittagong, Chattagram, Bangladesh
  • Velaga Appala Raju Department of Medicinal Chemistry, Faculty of Pharmacy, MAHSA University, Kuala Langat, Selangor, Malaysia
  • Imtiaz Khalid Mohammed Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Cyberjaya, Selangor, 63000 Malaysia

DOI:

https://doi.org/10.5530/ctbp.2024.3s.15%20

Keywords:

Ethanol, Pterospermum semisagittatum, phytochemicals, cytotoxic and antidepressant

Abstract

This report investigates the phytochemical, cytotoxic and antidepressant property of the ethanolic extract of Pterospermum semisagittatum from South Asia region. Extracts subjected to pharmacological activity using in vitro and in vivo methods which are cytotoxic and antidepressants activity on animal model alongside its phytochemical profiling. The cytotoxic activity was investigated using brine shrimp lethality assay and antidepressant activity was determined by forced swimming test (FST) and tail suspension test (TST) in mice. The plant extract of ethanol extract of Pterospermum semisagittatum (EEPS) was found secondary metabolites, notably steroids, glycosides, tannins, flavonoids, saponins, and alkaloids etc. EEPS showed substantial cytotoxic effects lethal concentrations (LC50) value are 347.65 μg/mL. Besides, treatment with EEPS revealed a significant reduction of immobility time in a dose-dependent manner in Force Swimming Test (FST) and Tail Suspension Test (TST). The dose of 400 mg/kg, body weight shows the best antidepressant effects in both FST and TST which are respectively immobile time 198.5± 3.81**** and 157.5 ± 4.01**** statistically significant (****P < 0.0001). Subsequently, the dose of 200 mg/kg, body weight shows the best antidepressant effects in both FST and TST which are respectively immobile time 180± 3.03**** and 33.75± 2.95**** (statistically significant).The investigation concludes that EEPS can be a potent source of antidepressant and cytotoxic agents

Cytotoxicity activity of EEPS

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Published

02-10-2024

How to Cite

Mohammad Hamid Ullah, Masro Mohamad, Shamima Abdul Rahman, Mohammed Abu Sayeed, Velaga Appala Raju, & Imtiaz Khalid Mohammed. (2024). The cytotoxic and anti-depressant activity of ethanol extract of Pterospermum semisaggitatum (buch. ham.exroxb.) leaves: an in vitro and in vivo study . Current Trends in Biotechnology and Pharmacy, 18(3s), 200–208. https://doi.org/10.5530/ctbp.2024.3s.15