Formulation and Development of Poly-Herbal Anti-ageing Cream

Abstract

Skin ageing is a complicated biological process that is affected by both intrinsic and extrinsic factors. UV rays produce free radicals that can impair the collagen and elastin networks. Consequently, the cell regeneration process is impeded, resulting in hyperpigmentation and rough, wrinkled skin. As a result, the research advances in natural cosmetics that contribute to restoring a certain level of rejuvenation and delaying or reversing the ageing process are the focus of the study. The purpose of this research is to determine the percentage of radical scavenging activity of aqueous extract of Tremella fuciformis (TF) using the DPPH technique, as well as to develop a polyherbal anti-ageing cream containing TF, sea buckthorn, grape seed, rosehip seed, rose water, and natural excipients. The maceration technique was employed in the preparation of the TF extract. Hot water bath equipment was used to prepare oil-in-water emulsions by heating the aqueous phase and oil phase components separately at 75 °C. Subsequently, mixing both phases with the use of a homogenizer set at 1000 rpm for 5 minutes and reduced to 500 rpm until the emulsion cooled down to room temperature was necessary. Following that, the emulsion cream-based was levigated with TF extract to produce a smooth small amount of herbal cream, which was gradually incorporated with a large portion of the cream base until forming a homogenous polyherbal cream. At 2 mg/ml concentration, the TF extract demonstrated 80% radical scavenging activity. The polyherbal cream appeared light yellow with a smooth texture and was homogenous without phase separation. The formulation showed excellent stability across the 3 months of testing in different temperatures and different packaging materials, including plastic and glass containers. From the above results, it is concluded that the formulation containing TF could be the best formulation to rejuvenate the skin and reduce age-related wrinkles.