Isolation and characterization of Gram-positive and Gram-negative Bacterial Membrane Vesicles using Poly Ethylene Glycol Method

Authors

  • Huldah Lazarus School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India
  • Swetha Vasudevan School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India
  • Niha Farooque School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India
  • Nalini Easwaran School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India

DOI:

https://doi.org/10.5530/ctbp.2023.3s.64

Keywords:

Membrane vesicles, Isolation, Characterization, Lactobacillus acidophilus, Pseudomonas syringae

Abstract

Membrane vesicle secretion was predicted earlier as a method of elimination of proteins, lipids, and RNA from the cell. At present vesicles are thought to be a brand-new type of intercellular communication and they are playing a crucial role in numerous physiological and pathological processes. However, in this expanding field, much remains unknown regarding the origin, biogenesis, secretion, target and fate of these vesicles. Vesicle research might become a more established field as a result of a complete understanding of the isolation and analysis techniques currently in use, making it possible to utilize membrane vesicles in therapeutic settings. Thus, standardization of the isolation of vesicles is in great need. This research article deals with the isolation and characterization of two distinct bacterial species-derived membrane vesicles namely, Lactobacillus acidophilus a human gut Bacterial MVs and Pseudomonas syringae, a model phytopathogen.

Visualization of biofilm (Qualitative assay) under SEM: a. Biofilm of L. acidophilus and b. P. syringae

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Published

31-10-2023

How to Cite

Lazarus, H., Vasudevan, S. ., Farooque, N. ., & Easwaran, N. . (2023). Isolation and characterization of Gram-positive and Gram-negative Bacterial Membrane Vesicles using Poly Ethylene Glycol Method. Current Trends in Biotechnology and Pharmacy, 17(Supplement 3B), 1293–1302. https://doi.org/10.5530/ctbp.2023.3s.64