Preparation, Characterization, and In Vitro Release Study of Resveratrol-Laminarin-Loaded Liposomal Formulation

Abstract

<p style="text-align: justify;">The present study aims to formulate and evaluate the physicochemical characteristics of the resveratrol-laminarin encapsulated liposomal formulation. Resveratrol is a phytoalexin with numerous biological effects like antidiabetic, anti-oxidant, anti-cancer, and anti- inflammatory properties. A polysaccharide extracted from brown algae called laminarin possesses anti-tumor, anti-oxidant, anti-coagulant, anti-inflammatory, and antiobesity properties. Resveratrol, a hydrophobic drug, is entrapped in the liposomal bilayer, and laminarin, a hydrophilic drug, is enclosed in the hydrophilic core. The preparation and characteristics of resveratrol- laminarin-loaded liposomes, including their particle size, zeta potential, entrapment efficiency, and in-vitro release kinetics, were investigated in this study. The thin film lipid hydration method was used to prepare the liposome using soy phospholipids and cholesterol in a 6:1 ratio. Downsizing of the prepared liposomes was carried out by homogenization and sonication techniques. The liposome had a particle size of 120.7 nm after sonication, and a zeta potential of -33.4 mV was observed. The highest entrapment efficiency recorded for resveratrol is 90.69%, and 83.64% for laminarin. Biophysical characterisation of the prepared liposomes was carried out by scanning electron microscope and UV-visible spectrophotometry. The release study of resveratrol and laminarin was observed for six hours. A stable and steady release of the compounds was observed. The stability of the liposomes stored at 4&ordm;C was analyzed after three months of preparation. In conclusion, the prepared liposomal formulations exhibited good physicochemical characteristics and are of great therapeutic value in the future.</p>