Genetic Polymorphism of CD18 gene in Karan Fries Young bull Calves using PCR-RFLP Analysis

Order of Publishing in Issue: 
Volume :4
Issue :4
October, 2010
Page No: 
Yathish H.M*[1], Ashwani Sharma[1], Vijay Kumar[1], Asit Jain[1],
Dibyendu Chakraborty[1], Avtar Singh [1], M. S. Tantia2 and B. K. Joshi[2]
1National Dairy Research Institute (NDRI), Karnal, Haryana-132 001, India.
2National Bureau of Animal Genetic Resources, Karnal-132 001, India.

CD18 gene encodes the common subunitof b2 integrin molecules that are responsible forthe leukocytes to migrate into the site ofinflammation. Point mutation from adenine toguanine at 383 position of CD18 gene causes theloss of CD18 & CD11 subunits aggregation abilityand which leads to the Bovine LeukocyteAdhesion Deficiency (BLAD) syndrome. BLADis an autosomal recessive congenital disease ofHolstein Friesian (HF) cattle breed and ischaracterized by recurrent bacterial infections,delayed wound healing, stunted growth andpersistent marked neutrophilia. BLAD syndromewas spread throughout the world mainly by theuse of semen from carrier animals in artificialinsemination (AI) as they have viability. Use ofHF bulls or their semen extensively forcrossbreeding programmes during the last fivedecades made the screening of farm born HFand its crossbreds mandatory before their use inbreeding programmes. In the present study,isolated genomic DNA from young Karan Friesbull calves was amplified and PCR products weresubjected to RFLP analysis using Taq I restrictionenzymes. Result indicated that out of 55 examinedcalves, 2 (3.64%) were BLAD carriers (BL/TL)and 1 (1.82%) was BLAD affected (BL/BL).This study has recommended the nation to screenall HF and its crosses across the country forBLAD.

CD18, BLAD, Karan Fries, Congenital Disease, PCR-RFLP.
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