Deciphering the Biological Activity of Recombinant Guinea Pig Proteins

Order of Publishing in Issue: 
Volume :14
Issue :1
January, 2020 - March, 2020
Page No: 
Madhavan Omanakuttan[1], Vijaya R. Dirisala[1],*, Hanumohan R. Konatham[1], M. Madhuri[2], Malathi Jojula[2], Shradha Mawatwal[3], Rohan Dhiman[3]
[1]Department of Biotechnology, Vignan’s University, Guntur-522213, AP, India
[2]Department of Microbiology, Sri Shivani College of Pharmacy, Warangal - 506001, Telangana, India
[3]Laboratory of Mycobacterial Immunology, Department of Life Science, National Institute of Technology, Rourkela–769008, Odisha, India

The guinea pig is one of the most relevant small animal models for studying a large number of infectious and non-infectious diseases. The major roadblock for its effective utilization is the lack of readily available molecular and immunological reagents. In order to address this issue, some of the important recombinant proteins such as IL-4, IL-10, IL-1β, MCP-1, IFN- y and TNF- α which were generated previously were analyzed for their biological activity in terms of nitrite production in RAW cells. Interestingly, nitrite production was comparatively more in the presence of TNF-α in comparison with proinflammatory cytokine IFN-y and similar levels of nitrite production were observed in the presence of Th2 cytokines i.e IL-4 and IL-10. We have analyzed the biological activities of these recombinant proteins so that they could be used for a wide array of in vitro and in vivo biological assays.

Th1, Th2, Guinea pig, Assay, Proteins.
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