In vitro Propagation for Rapid and Efficient Regeneration of Cardiospermum halicacabum L. – An important Medicinal Plant

Order of Publishing in Issue: 
9
Volume :10
Issue :4
October, 2016
Page No: 
368-373
Authors: 
Ashwani Kumar [1, 2]*, S.C. Goyal [1], Pooja [1], Jagdish Parshad [3], Anshuman Singh [2], Parvender Sheoran [2], Charu Lata [2], Rajkumar [2] and Meenakshi Jain [4]
Address: 
Department of Botany & Plant Physiology [1], Department of Microbiology [3], Department of Molecular Biology and Biotechnology [4], CCS HAU, Hisar – 125004, Haryana, India
Address: 
[2] ICAR-Central Soil Salinity Research Institute, Karnal-132001, Haryana, India

Abstract

Cardiospermum halicacabum, a medicinial plant rich in β-sitosterol, Dglucoside, saponin and quebrachitol. Direct regeneration was achieved only from the nodal explants which showed 100 per cent sprouting on MS medium supplemented with BAP (0.5 ppm) + NAA (1.0 ppm) in 12 days, while MS medium supplemented with BAP (0.5 ppm) + IBA (1, 2 or 3 ppm) showed early but decreased sprouting. The half strength MS basal medium without any growth regulator proved to be best medium for root induction for excised shoots. The in vitro raised plantlets were transplanted to mixture of sterile dune sand and FYM (3:1) with moderate (80 %) success.

Key words: Cardiospermum halicacabum, nodal explants, axillary buds, growth regulators.

Keywords: 
Cardiospermum halicacabum, nodal explants, axillary buds, growth regulators.
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