In vitro Antibacterial Activity in Seed extracts of Phoenix sylvestris Roxb (Palmae), and Tricosanthes dioica L (Cucurbitaceae)

Order of Publishing in Issue: 
3
Volume :5
Issue :1
January, 2011
Page No: 
993
Page No: 
997
Authors: 
Vijay Kothari
Address: 
Institute of Science, Nirma University, Ahmedabad-382481, India.

Seeds of Phoenix sylvestris Roxb (Palmae), and Tricosanthes dioica L (Cucurbitaceae) were investigated for their in vitro antibacterial activity against few gramnegative and gram-positive bacteria. Extracts of the plant seeds were prepared in methanol, ethanol, and chloroform by microwave assisted extraction method. Their antibacterial activity was investigated by disc diffusion and broth dilution methods. Ethanol extract of P. sylvestris was found active (bacteriostatic) against both grampositive and gram-negative organisms with MIC (minimum inhibitory concentration) values of 481 and 410 ?g/mL against Salmonella paratyphi A and Staphylococcus epidermidis, respectively. Phytochemical screening revealed presence of phenols, alkaloids, and flavones in it. This study indicated various extracts of P. sylvestris, and T.dioica seeds to possess antibacterial activity.

Keywords: 
Antibacterial, Phoenix sylvestris, Tricosanthes dioica, MAE (microwave assisted extraction)
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