Use of Soybean Oil Fry Waste for Economical Biosurfactant Production by Isolated Pseudomonas aeruginosa Using Response Surface Methodology

Order of Publishing in Issue: 
6
Volume :3
Issue :2
April, 2009
Page No: 
162-171
Authors: 
C. J. B. de Lima and J. Contiero*
Address: 
Department of Biochemistry and Microbiology Biological Sciences Institute, Rio Claro, São Paulo, University - Unesp - CEP 13506-900 Rio Claro, São Paulo, Brazil
Email-ID: 
jconti@rc.unesp.br


Abstract:
he present study sought biotensoactiveproduction from soybean oil fry waste usingPseudomonas aeruginosa ATCC 10145 andPseudomonas aeruginosa isolated from the soilof a petroleum station having undergone gasolineand diesel oil spills. The results of the experimentswere analyzed using a complete factorialexperimental design, investigating theconcentration of soybean oil waste, ammoniasulfate and residual brewery yeast. Assays wereperformed in 250-mL Erlenmeyer beakerscontaining 50 mL of production medium,maintained on a rotary shaker at 200 rpm and atemperature of 30±1 °C for a 48-hourfermentation period. Biosurfactant productionwas monitored through the determination ofrhamnose, surface tension and emulsificationactivity. The Pseudomonas aeruginosa ATCC10145 strain and isolated Pseudomonasaeruginosa were able to reduce the surfacetension of the initial medium from 61 mN/m to32.5 mN/m and 30.0 mN/m as well as producerhamnose at concentrations of 1.96 and 2.89 g/Lwith emulsification indices of 96% and 100%,respectively.

Keywords: 
Pseudomonas aeruginosa, Biosurfactant, Rhamnose, Surface-active, Emulsification index, Soybean oil.
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