Targeted Integration of Bacillus thuringiensis Sigma-Endotoxin cry1Fa1 in Brinjal (Solanum melongena L.)

Order of Publishing in Issue: 
8
Volume :5
Issue :2
April, 2011
Page No: 
1149-1156
Authors: 
Dipty Shrivastava,[1] Monika Dalal, [1]$ Vikrant Nain,[1]# P. C. Sharma, [2] P. Ananda Kumar*[1]
Address: 
[1] National Research Centre on Plant Biotechnology, IARI, New Delhi -110012, India.
Address: 
[2] School of Biotechnology, GGS Indraprastha University, Sector -16C Dwarka Delhi, India.
Address: 
#School of Biotechnology, Gautam Buddha University, Greater Noida-201308, India
Address: 
$National Research Centre for Sorghum, Rajendra Nagar, Hyderabad-500 030, India
Email-ID: 
kumarpa@nrcpb.org

Abstract : Gene integration in plants by homologous recombination eliminates variation in transgene expression and gives a predictable plantphenotype. Present study demonstrates homologous recombination-mediated integration of Bacillus thuringenisis cry1Fa1 gene atFlavonoid-3-glucosyltransferase (anthocyanin biosynthesis pathway) locus in Brinjal (Solanum melongena L.), for introduction of resistanceagainst its major insect pest ‘Brinjal Fruit and Shoot Borer’ (BFSB) (Leucinodes orbonalis). The transgenic lines showed complete protectionagainst BFSB. These results provide a novel perspective on development of genetically modified crops with stable and predictabletransgene expression, and the possibility of the utilization of such promising technology in future crop improvement programmes.

Keywords: 
Anthocyanin, Bt transgenic, Gene targeting, Homologous recombination, cry1F, Leucinodes orbonalis.
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