Synthesis of Methyl Esters by Transesterification Catalyzed by Cutinase from Pseudomonas cepacia NRRL B 2320 and Kinetic analysis

Order of Publishing in Issue: 
Volume :8
Issue :1
January, 2014
Page No: 
Kasturi Dutta, Krishnamoorthy Hegde and Veeranki Venkata Dasu*
Biochemical Engineering Laboratory, Department of Biotechnology Indian Institute of Technology Guwahati Guwahati – 781 039, Assam, India

AbstractThe synthesis of methyl esters by transesterification of tributyrin, triolein and soyabean oil in organic solvent was demonstrated using cutinase from Pseudomonas cepacia NRRL B-2320. The optimal temperature range for the enzyme catalyzed synthesis was found to be from 35°C to 40°C. The maximum conversion (65%) during synthesis of ester was obtained with 2.5 mg/ml of enzyme. The optimal methanol to oil ratio was found to be 1.5:1 with all three substrates. The kinetic analysis for the synthesis of methyl esters from tributyrin/triolein/ soyabean oil have shown that the Ping-Pong bi bi model with alcohol inhibition was found to be the most suitable for the prediction of the reaction kinetics for all the cases.

Biodiesel, Cutinase, Ping-Pong bi bi model, Pseudomonas cepacia, Transesterification.
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