Studies on the Production of Actinomycin by Nocardioides luteus, a Novel Source

Order of Publishing in Issue: 
Volume :5
Issue :3
July, 2011
Page No: 
El-Refai, H.A.[1]*, AbdElRahman [1], H.Y., Abdulla, H.[2], Hanna, Atef G. [1], Hashem, A.H. [3], El-Refai A.H. [1] and Ahmed, E.M.[1]
[1] Chemistry of Natural and Microbial Products Dept., National Research Center, Dokki, Cairo, Egypt
[2] Dept. of Botany, Faculty of Science, Suez Canal University, Egypt
[3] Dept. of Microbiology, Faculty of Pharmacy, Cairo University, Egypt

Abstract: Efforts have been devoted to explore the antimicrobial activity of local egyptian actinomycetes isolates. 127 pure actinomycetesisolates were recovered from the stones at the location of the tomb of Tell Basta (the site of the ruins of the old temple of Bast) which is southeastof the Nile Delta at the township of Zagazig in Egypt. The most competent isolate showed promising activity against some pathogenicyeasts, fungi and bacteria. It was identified as Nocardioides luteus. Using 2 ml of 2 days old inoculum of Nocardioides luteus in fermentationmedium consisting of (g/l): starch, 10; yeast extract, 4; NaCl, 10; K2HPO4, 2; MgSO4.7H2O, 0.05; CaCO3, 0.02 and FeSO4.7H2O, 0.01,adjusted before autoclaving at pH 7 and incubated for 7 days with shaking rate 200 rpm resulted in maximum antimicrobial activityagainst Candida albicans ATCC 10231. The minimum inhibitory concentration (MIC) of the culture was determined to be 0.4 ?g/ml. Trialshave been conducted to separate and identify the active compound(s) responsible for the  anticandidal activity. Based on the spectroscopicdata from UV, IR, 1H-NMR and 13C-NMR spectra, the isolated compounds were identified as derivatives of actinomycins class. To ourknowledge, this is the first time not only to study systematically the bioactivities of Nocardioides luteus; but also to report actinomycin productionby a Nocardioides spp. Cytotoxicity of both the culture filtrate extract and the impure compound were tested against different tumor cell lineswhere they showed remarkable cytotoxicity against all challenged cell lines.

Actinomycetes, antimicrobial, actinomycin, cytotoxicity.
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