Studies on Carrier State of Chlamydophila abortus in Naturally Infected Sheep

Order of Publishing in Issue: 
5
Volume :5
Issue :2
April, 2011
Page No: 
1123-1129
Authors: 
Rabia Abadía Elzlitne[1], Giuma Elaref Elhafi [2], Vijayan R [1] and Sateesh Kumar [1]*
Address: 
[1] Faculty of Vet. Medicine, Al Fateh University, Tripoli, Libya.
Address: 
[2] Animal Health Center Tripoli, Libya
Email-ID: 
yeshjain@gmail.com

Abstract: The purpose of the study was to establish whether or not C. abortus is shed through the reproductive tract following infection undernatural conditions. Two farms where enzootic abortion was endemic were investigated by sequential collection of vaginal secretions, faecesand serum samples from ewes which had chlamydial abortion six months earlier.PCR was used as a confirmatory tool after extracting DNAfrom faeces and vaginal secretions‘ using primers specific to the MOMP gene .None of the samples tested were positive, suggesting that either therewere no EBs of C. abortus in the samples or there were non infectious forms (RBs) which could not be detected by DNA amplification.Real-time PCR analysis of placental samples identified very few or no chlamydial genomes, which contrasted significantly with samples takenat the time of abortion. The C. abortus was detected by PCR for the first time in milk samples collected from ewes with EAE history using theIMS technique. The serum samples from these ewes showed significantly higher chlamydiaspecific IgG titre during the period of periovulation.In terms of flock management, the products of abortion should be considered the major and principal source of infection fortransmission to naïve ewes.

Keywords: 
Chlamydophila abortus, Sheep
AttachmentSize
Paper-5.pdf475.48 KB

 
To download full text articles and issues in PDF at no cost.All you have to do is fill out a brief registration form. It's easy and it's free. At any time, you may request removal of your contact information from our database.
Click here to Register | Click here if you have already registered

2018 ABAP Awards

Invitation for Nominations to 2019 ABAP Awards
The Association of Biotechnology and Pharmacy invites nominations for the following awards from the eligible talented people with proven track record and achievements on or before August 31, 2019 in the prescribed format.

DOWNLOAD CIRCULAR | APPLICATION FORM

AWARDS

User login