Statistical optimization of PolyHydroxy Butyrate (PHB) production by novel Acinetobacter nosocomialis RR20 strain using Response Surface Methodology

Order of Publishing in Issue: 
7
Volume :14
Issue :1
January, 2020 - March, 2020
Page No: 
62-69
Authors: 
Ranganadha Reddy A[1], Vidya Prabhakar K[2], Venkateswarulu TC[1], Krupanidhi S[1], Md. Nazneen Bobby, Abraham Peele K, P. Sudhakar[3], P. Vijetha[4]
Address: 
[1]Department of Biotechnology, VFSTR University, Guntur, Andhra Pradesh, India 522213
Address: 
[2]Department of Biotechnology, VS University, SPSR Nellore, Andhra Pradesh, India 524320
Address: 
[3]Department of Biotechnology, ANU University, Guntur, Andhra Pradesh, India 522510
Address: 
[4]Department of Chemical Engineering, VFSTR University, Guntur, Andhra Pradesh, India 522213
Email-ID: 
arr_bt@vignan.ac.in
Email-ID: 
rangaaluri@gmail.com

Eco-friendly biopolymers, polyhydroxybutyrate, have been produced by many kinds of bacteria that possess most important applications in food packaging industries and also in medical field. In the present work, PHB production was economized through the statistical optimization of physical variables. The physical variables chosen for the enhanced production of PHB were incubation period, temperature, pH and inoculums size. The relative rate of production has been studied by understanding the complex interactions of variables using face-centred central composite design. The submerged fermentation with Acinetobacter nosocomialis RR20 produced PHB yield of 5.88 g/L at optimized conditions with a notable value change of sixfold increase in comparison with minimal salt medium and these findings have showed that the designed medium was significant in terms of higher of PHB production.

Keywords: 
Polyhydroxybutyrate, Acinetobacter nosocomialis RR20 strain, Response surface methodology, Shake flask culture.
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