Statistical Optimization of Anti-leukemic Enzyme L-Asparaginase Production by Penicillium cyclopium

Order of Publishing in Issue: 
3
Volume :8
Issue :2
April, 2014
Page No: 
130-142
Authors: 
Heba A. El-Refai[1]*, Mona S. El-Shafei[1], Hanan Mostafa[1], Abdel-Monem H.El-Refai[1], Fawkia M. El-Beih[2], Ghada E.A. Awad[1], Saadia M.Easa[2] and Sanaa.K.Gomaa[1]
Address: 
[1]Chemistry of Natural and Microbial Products Department, National Research Center, Dokki, Cairo 12622, Egypt
Address: 
[2]Ein-Shams University , Faculty of Science, Microbiology Department, Cairo, Egypt.
Email-ID: 
dr.heba_ar@yahoo.co

A sequential optimization strategy, based on statistical experimental designs is employed to enhance L-Asparaginase production. First, a 2-level Plackett-Burman design was applied for bioprocess parameters screen that significantly influence the enzyme production. Seven culture ingredients and fermentation period were examined for their significance on enzyme production and specific activity using Plackett– Burman factorial design. MgSO4.7 H2O, KCl and (NH4)2SO4 were the most significant factors improving enzyme production process. The second optimization step was performed using fractional factorial design in order to optimize the amounts of highest positive variables had significant effect on enzyme activity. The second optimization step was to identify optimal values of the three factors that bringing about maximum L-asparaginase activity, using central composite designed experiments. The different media components were initially optimized using the conventional one-factor-at-a time method. Lasparaginase activity in the optimized medium was 160 IU mL-1 which increased 1.54-fold over that of the conventional one-factor-at-a time method basal medium (104.5 ± 1.6 IU mL-1).

Keywords: 
L-asparaginase, Plackett-Burman design, Response surface methodology, central composite design, Penicillium cyclopium.
AttachmentSize
Paper-3.pdf665.96 KB

 
To download full text articles and issues in PDF at no cost.All you have to do is fill out a brief registration form. It's easy and it's free. At any time, you may request removal of your contact information from our database.
Click here to Register | Click here if you have already registered

2018 ABAP Awards

Invitation for Nominations to 2019 ABAP Awards
The Association of Biotechnology and Pharmacy invites nominations for the following awards from the eligible talented people with proven track record and achievements on or before August 31, 2019 in the prescribed format.

DOWNLOAD CIRCULAR | APPLICATION FORM

AWARDS

User login