Spectroscopic studies, Antioxidant and Anticancer attributes of diffusible eumelanin produced by marine Streptomyces rochei

Order of Publishing in Issue: 
4
Volume :12
Issue :2
April, 2018 - June, 2018
Page No: 
147-158
Authors: 
Satheesh Periyasamy[1], Shalini Devi.S[2], Preethi Kathirvel[1]
Address: 
[1]Department of Microbial Biotechnology, Bharathiar University, Coimbatore, India.
Address: 
[2]Department of microbiology, Bhavans Vivekananda college, Hyderabad, India

The diffusible melanin pigment from marine Streptomyces rochei isolated from Vishakhapatnam coast was characterized using UV Spectrophotometer, GC-MS, NMR, EPR Spectroscopy, CHNS elemental analysis, Ramon Spectroscopy and found to be a pure form of eumelanin. Anticancer activity of the melanin pigment from Streptomyces rochei was studied by MTT assay using MFB4 cell lines with an IC50 value of 26 μg/mL, in comparison the standard 5- fluorouracil exhibited an IC50 value of 16.5 μg/mL and the DNA damage was also assessed by alkaline single-cell gel electrophoresis (COMET assay). The antioxidant activity was assessed by DPPH assay and the percentage of inhibition at 100 μg/mL concentration of melanin was found to be 71.53 which was higher than that for 5- fluorouracil at 63.32 and ascorbic acid at 51.43. The potential for commercial exploitation of this melanin for its biotechnological role is enormous and the Streptomyces rochei strain can be a credible source of melanin and can serve as cost effective substitute for the currently marketed products.

Keywords: 
Eumelanin, Spectroscopy, MTT, DPPH, COMET.
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