Simple, Rapid and Sensitive Method for Determination of Nitrofurantoin in Human Plasma by using Liquid Chromatography / Tandem Mass Spectrometry

Order of Publishing in Issue: 
7
Volume :6
Issue :2
April, 2012
Page No: 
196-203
Authors: 
Rajaram S. Patil[1]*, Chaitanya Krishna, A [2]., P. V.D.L.S.Ravi Prakash [2] and Sangita R. Patil [2]
Address: 
[1] Thermofisher Scientific India Pvt. Ltd. Mumbai, India
Address: 
[2] Quest life sciences Private Ltd, Chennai, India
Email-ID: 
drrsp1@rediffmail.com

Abstract
A simple, rapid and sensitive method using an isocratic Liquid chromatography coupled with Tandem mass spectrometry was developed and validated for the assay of nitrofurantoin in the Human Plasma. The Mass transition of nitrofurantoin and losartan (Internal standard) were M/z 237.000/151.800 and M/Z 421.300/ 179.274 in ESI Negative ionization. Linearity was observed between the nitrofurantoin concentration and the peak area ratio from 10.107 to 999.900ng/mL with r2 value of 0.99. Plasma samples containing nitrofurantoin were extracted with ethyl acetate. The observed recovery of nitrofurantoin was 80.2 %. The intraday and inter-day accuracy ranged from 83.61 to 107.16% and from 93.13to 103.02% respectively, at Low, middle and high level concentrations. The method will be used in the determination of the pharmacokinetic parameters of nitrofurantoin after oral administration of nitrofurantoin formulation in human Plasma.

Keywords: 
Nitrofurantoin, LCMS/MS, Bioanalytical, Validation, Human Plasma, 5- nitrofurans
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