Simple Approach to Reactive Dye Decolorization Using Trichosanthes dioica Proteins at Low Concentration of 1-hydroxybenzotriazole

Order of Publishing in Issue: 
4
Volume :5
Issue :3
July, 2011
Page No: 
1273 -1281
Authors: 
Farrukh Jamal
Address: 
Department of Biochemistry, Dr. Ram Manohar Lohia Avadh University, Faizabad-224001, U.P., India

Enzymatic catalysis in the presence of redox mediators has emerged as an effective and feasible technique for degradation of complexstructural compounds. We investigated peroxidase from Trichosanthes dioica to study decolorization of reactive dyes namely ReactiveBlue15 (RB15) and Reactive Red4 (RR4) under different experimental conditions like pH, temperature, time interval, enzyme concentrationand in the presence of redox mediators. Six different redox mediators; syringaldehyde, guaiacol, 1-hydroxy-benzotriazole (HOBT),vanillin, bromophenol and quinol were simultaneously evaluated. T. dioica peroxidase showed remarkable decolorization of reactivedye in the presence of 1-hydroxybenzotriazole. At an enzyme concentration of 0.45 EUmL-1 the peroxidase decolorized Reactive Red15 almostcompletely up to a maximum of 98.6% whereas Reactive Red4 decolorized upto 68.2% with 1.0 mM 1-hydroxybenzotriazole. Maximumdecolorization was recorded at a temperature range of 40°C to 50°C at pH 5.0. Time activity plot exhibited maximum decolorization at 90 minand 180min for RB15 and RR4 respectively. It can be concluded that T. dioica peroxidase could be a potential source for developing aninexpensive and efficient method for the treatment of recalcitrant reactive dyes that are potentially toxic or even carcinogenic.

Keywords: 
Reactive Dyes; 1-hydroxybenzotriazole; Decolorization; Trichosanthes dioica Peroxidase
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