In-silico Prediction of T and B cell Epitopes in the Evolutionary Conserved Pathway of Glycolysis for Human Pathogens: Coccidiodes immitis, Histoplasma capsulatum and Pneumocystis carnii

Order of Publishing in Issue: 
2
Volume :11
Issue :3
July, 2017 - September, 2017
Page No: 
242-252
Authors: 
Neelabh and Karuna Singh*
Address: 
Department of Zoology, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi-221005, India

Fungal diseases are amongst the emerging diseases to which humans are most susceptible pertaining to the present day life style. New drugs are being made but at a slow pace, not matching the resistance developing capacity of the fungal pathogens. Therefore, it is important to choose new drug targets peculiar to fungi but absent in humans.A common practice till date has been to use the virulence proteins in order to devise medicines against micro-organisms. However, we have used in-silico techniques to analyze enzymes involved in the evolutionary conserved pathway of glycolysis which is the most primitive pathway for ATP production in aerobic as well as anaerobic organisms. Therefore, on successfully targeting these enzymes microorganism can be killed. We have chosen 3 fungal pathogens viz.Coccidiodes immitis, Histoplasma capsulatum and Pneumocystis carnii that cause serious diseases in human beings and their effect on the morbidity and mortality of humans has been substantial. MEME-Motif discovery tool was used to devise a single motif sequence per glycolytic enzyme from the three fungi. Further, online servers such as ABCpred, Bcepred, BepiPred and Tools from Immunomedicine group have been used in order to predict the linear epitopes from the motif sequences of the different glycolytic enzymes. Analysis of these epitopes was performed through PepCalc and NetSurfP in terms of parameters such as hydrophilicity, coil forming residues and exposed residues. Analysis of ten proteins of glycolysis from each fungus reveals the regions that can elicit an immunological response. This study most importantly projects aldolase to be an enzyme of importance which in future can be used as a potential vaccine target for these three fungi.

Keywords: 
In-silico, Epitope, Motif, Glycolysis, Fungi.
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