Seroprevalence of Antibodies to HPV L1 in a Limited Population study determined by the GST-capture ELISA

Order of Publishing in Issue: 
1
Volume :9
Issue :2
April, 2015
Page No: 
97-106
Authors: 
Ravi Ranjan Verma [a] , Rajan Sriraman [b], NM Ponnanna [c], Samir Kumar Rana [c], Shikha Srivastava [d], JK Roy [d], Sadhana Gupta [e], M Madhanmohan [a] and V A Srinivasan [c]*.
Address: 
[a] Department of Biotechnology, Jawaharlal Nehru Technological University Hyderabad and R&D Centre, Indian Immunologicals Ltd., Gachibowli P.O., Hyderabad 500032, India.
Address: 
[b] B13/166 Sonarpura, Varanasi 221001, India
Address: 
[c] National Dairy Development Board, R&D Laboratory, Gachibowli P.O., Hyderabad 500032, India.
Address: 
[d] Department of Zoology, Banaras Hindu University, Varanasi 221005, India.
Address: 
[e] Department of Obstetrics & Gynaecology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.

Human papillomavirus (HPV) infection elicits an antibody response in most individuals, mainly to L1- the major capsid protein of the virus. The Glutathione S-Transferase (GST) capture ELISA was evaluated in a limited population study (n=187 women donors) for its suitability in determining the seroprevalence of antibodies specific to L1 of five high-risk HPV genotypes (16, 18, 31, 45 and 52). Serum samples were obtained from some of the hospital-visiting women aged between 18-60 years with an unknown history and/or status of infection. Most samples (42%) showed sero-positivity to HPV 45 (80/187) followed by sero-positivity to HPV 16 (32%), 18 (16.5%), 52 (10%) and a single (0.5%) positive sample for HPV 31. There was significant cross-reactivity in the samples across the assayed genotypes. The results suggest that the GST-capture ELISA might serve as an efficient alternative to VLP-ELISA in sero-epidemiological studies especially in the large-scale, preliminary, screening of individuals hitherto untested for HPV infection.

Keywords: 
PV genotype 16, 18, 31, 45 and 52; GST-L1; GST-capture ELISA; Sero-positivity; VLP-ELISA
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