Sequential Optimization of Production of Cephamycin C Using Nocardia lactamdurans: Effect of Nutritional Supplements, Metabolic Precursors and Inducers

Order of Publishing in Issue: 
9
Volume :5
Issue :3
July, 2011
Page No: 
1325-1337
Authors: 
Lalit D. Kagliwal*, Shrikant A. Survase and Rekha S. Singhal
Address: 
Food Engineering and Technology Department, Institute of Chemical Technology Matunga, Mumbai 400 019, India

Abstract : In this study, Nocardia lactamdurans NRRL 3802 was optimized for the first time for production of cephamycin C by using submergedfermentation. The effects of various carbon source, nitrogen source, inoculum size, initial pH of culture medium, minerals and amino acidswere investigated for the maximum production of cephamycin C by N. lactamdurans NRRL 3802 in submerged fermentation. Subsequently,selected fermentation parameters were further optimized by Taguchi orthogonal array method and response surface methodology (RSM).Glycerol and yeast extract as carbon and nitrogen sources with initial pH of culture medium of 5.5 and inoculum size of 109 CFU/ml at 28 ± 2 °Cafter 3 days gave maximum production of 3042.19 ± 62.36 mg/l of cephamycin C as compared to 243.03 ± 9.43 mg/l beforeoptimization. Incorporation of 1,3- diaminopropane at 1.2 % v/v in RSM optimized medium further increased the yield to 3973.20 ±57.96 mg/l.

Keywords: 
Cephamycin C, Nocardia lactamdurans, Inducer, Taguchi, RSM
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