Acorus calamus L., of Araceae is an aromatic herb, indigenous to Central Asia and EasternEurope. The fragrant oil obtained by alcoholic extraction of the rhizome is mainly used inthe pharmaceutical and oenological industries. Nevertheless, the occurrence of ?-asarone [(Z)-1, 2, 4-trimethoxy-5-prop-1-enyl-benzene] limits the possibility of its use due to its carcinogenicproperties. The ?-asarone content in A. calamus is dependant on the ploidy level of the plant. Theaim of the study is to distinguish between the diploid and tetraploid plants of A. calamuscontaining low and high ?-asarone respectively, using simple and easy to perform phytochemicalmethods. HPTLC profile of the alcoholic extracts of the diploid and tetraploid plants of A. calamusshowed visible and distinct variations. Therefore this method could be used to screen low and high?-asarone containing varieties of A.calamus.