Purification, Partial Characterization and Applications of Alkaline Protease from Bacillus subtilis NCIM No. 2724

Order of Publishing in Issue: 
4
Volume :7
Issue :3
July, 2013
Page No: 
732-742
Authors: 
Sujatha P. and Radhika P.*
Address: 
Department of Biochemistry, Andhra University, Visakhapatnam – 530 003, Andhra Pradesh, India

Abstract :

Thermostable alkaline protease production from Bacillus subtilis NCIM No. 2724, its purification and characterization was investigated. The purified enzyme revealed MW of 26 KDa. This enzyme is stable at a pH and temperature range of 7-11 with optimum pH at 10.0 and 30 to 70oC with optimum activity at 60oC, respectively. The enzyme is strongly activated by metal ions such as Ca2+, Mg2+ and Mn2+ while the activity is negatively regulated by EDTA, urea, guanidine hydrochloride and SDS. Whereas >50% activity of the purified enzyme was observed in the presence of commercial detergents tested, at 60oC. Blood stain removal was effective with this enzyme when supplemented with CaCl2 and glycine.

Keywords: 
Alkaline protease, Bacillus subtilis, characterization, detergent compatibility, purification, thermostability.
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