Protein Expression and Microscopic Evidence of White Spot Syndrome Virus (WSSV) in Tiger prawns Penaeus monodon

Order of Publishing in Issue: 
6
Volume :5
Issue :1
January, 2011
Page No: 
1011-1020
Authors: 
Bhatt, S.S.[a]*, Chavda, D. V.[a] and Sreepada, R. A. [b]
Address: 
[a] B.R. Doshi School of Biosciences, Zoology Laboratory, Bakrol Vadtal Road, Satellite Campus, Sardar Patel University, Vallabh Vidhyanagar 388120, India
Address: 
[b] National Institute of Oceanography, Dona Paula, Goa 403 004, India

Shrimp culture is being practiced in large areas of coastal farms of South Gujarat region in Western India.  The disease caused due to white spot syndrome virus (WSSV) has been detected in the cultured Penaeus monodon in this region. White spots were clearly visible in the carapace of infected shrimps. The  canning electron micrographs of the upper and lower surfaces of carapace revealed the presence of white spots measuring about 78?m to 135?m. In the infected shrimps, rod shaped viral particles have also been identified from the epidermis associated with carapace by TEM using negative staining. The histo-pathological analysis of gills, hepatopancreas, gut and muscles of P. monodon infected with WSSV indicated hypertrophied nuclei, presence of intranuclear inclusion bodies and chromatin margination in gill epithelial cells and necrosis of gill lamellae. In the infected muscles, necrosis, haemocytic infiltration and degeneration of muscle fibers were clearly evident. Damage to the mucosal epithelial cells of gut was accompanied by haemocytic inflammatory response. Inflammatory response and necrotic changes in hepatopancreas of infected shrimps have been observed. Hypertrophied and piknotic nuclei as well as infiltration of viral particles into connective tissues associated with vacuolization were observed in all the analyzed infected tissues. Changes in the expression of proteins have been detected by SDS-PAGE in the infected muscles and hepatopancreas. Four new proteins of 79kDa, 58kDa, 36kDa and 17kDa were found to be differentially expressed in muscles. In hepatopancreas, three proteins viz. 46kDa, 20kDa and 17kDa were differentially expressed in infected shrimps; whereas 62kDa protein, which was expressed in healthy shrimps, was not expressed in infected shrimps. No change in the protein expression pattern was detected in gills. The current study has shown that WSSV, besides causing damage to the tissues, has also  drastically altered the protein expression patterns of the infected tissues of P. monodon.

Keywords: 
WSSV, protein expression, Penaeus monodon, SDS-PAGE
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