The Protective role of Luffa acutangula fruit Methanolic Fraction against t-BHP Induced Oxidative damage in Human Erythrocytes

Order of Publishing in Issue: 
14
Volume :5
Issue :1
January, 2011
Page No: 
1073-1082
Authors: 
Boreddy Purushotham Reddy*, S. Venkata Mohan and P. N. Sarma
Address: 
Bioengineering and Environmental Centre, Indian Institute of Chemical Technology Hyderabad 500 607, India

The present study was designed to examine the ability of partially purified fractions of Luffa acutangula (Cucurbitaceae) fruit, to attenuate t-BHP induced oxidative damage in human erythrocyte as an in vitro model. Initially, we investigated the antioxidant property of the hexane, methanolic and aqueous extracts (FHE, FME and FAE, respectively) by DPPH free radical method and found that fruit methanolic extract was showing higher antioxidant activity (71.4±4.46% at 1 mg/ml) compared to other extracts (FHE&FAE were 13.93±1.3 and 51.84±3.76%, respectively). Hence, this extract was further partially purified chromatographically and out of these fractions (F1, F2, F3, F4, F2-1, F2-2, F2 -3 and F2-4) F2-3 showed significant antioxidant activity (73.96±6.4% at 25 ?g/ml). This fraction was further tested for its effect on lipid peroxidation, superoxide dismutase, catalase and glutathione in t-butyl hydroperoxide (t-BHP) treated-erythrocytes. Pretreatment with fraction F2-3 significantly inhibited lipid peroxidation in a dose and time dependent manner compared to control (40.6±3.2, 27.9±2.4 and 75.5±5.2 nmol MDA/gHb, at 30, 90 min and control, respectively). Catalase, SOD and GSH levels were also brought up in a dose and time dependant manner compared to control (treated and control were CAT: 100.7±4.7 and 51.3±3.2 ?MH2O2/ gHb/min, SOD: 9.68±0.87 and 1.15±0.12 IU/g Hb, GSH: 21.3±1.23 and 6.0±0.91 ?M/g Hb, respectively). These results establish that L. acutangula fruit aqueous fraction F2-3 possesses beneficial role in mitigating t-BHP induced oxidative stress in erythrocyte.

Keywords: 
Fruit methanolic extract; Reactive oxygen species , Superoxide anion, Hydroxyl radical; Malondialdehyde; Superoxide dismutase
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