Protective effects of in vitro supplementation of ascorbic acid on plasma membrane, acrosomal membrane and mitochondrial activity index of human spermatozoa

Order of Publishing in Issue: 
8
Volume :3
Issue :3
July, 2009
Page No: 
291 - 296,
Authors: 
Theng A.D., Gajare K.A.1and Deshmukh A.A.1*
Address: 
Department of Zoology, Govt. Vidarbha Institute of science and Humanities, Amravati, India
Address: 
1 Department of Zoology, Govt. Rajaram College, Kolhapur, India

AbstractSpermatozoa were the first type of cellsreported to produce free radicals. Reactiveoxygen species (ROS) mediated damage to spermis a significant contributing factor to maleinfertility. Impaired motility, impairedfertilization and oxidative DNA damage are threeinter-related mechanisms that account foroxidative stress mediated male infertility.Spermatozoa lack cytoplasmic antioxidantdefense due to exclusion of cytoplasm andtherefore rely upon antioxidants present in theseminal plasma. Centrifugation of a semensample prior to its use for intra-uterineinsemination (ICI) and in vitro Fertilization (IVF)induce oxidative stress. Therefore there is needto supplement the semen with antioxidants. Inthe present investigation attempts were made tostudy the effects of in vitro supplementation ofnon-enzymatic antioxidant ascorbic acid onsperm plasma membrane integrity, acrosomeintactness and mitochondrial activity index.There was highly significant (p<0.001)improvement in these parameters that relate tohealthy state of the spermatozoa.

Keywords: 
Spermatozoa, Oxidative stress, Male infertility, Antioxidants, Ascorbic acid
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