AbstractSpermatozoa were the first type of cellsreported to produce free radicals. Reactiveoxygen species (ROS) mediated damage to spermis a significant contributing factor to maleinfertility. Impaired motility, impairedfertilization and oxidative DNA damage are threeinter-related mechanisms that account foroxidative stress mediated male infertility.Spermatozoa lack cytoplasmic antioxidantdefense due to exclusion of cytoplasm andtherefore rely upon antioxidants present in theseminal plasma. Centrifugation of a semensample prior to its use for intra-uterineinsemination (ICI) and in vitro Fertilization (IVF)induce oxidative stress. Therefore there is needto supplement the semen with antioxidants. Inthe present investigation attempts were made tostudy the effects of in vitro supplementation ofnon-enzymatic antioxidant ascorbic acid onsperm plasma membrane integrity, acrosomeintactness and mitochondrial activity index.There was highly significant (p<0.001)improvement in these parameters that relate tohealthy state of the spermatozoa.