Protective effects of Calcium and Zinc on Lead induced perturbations in brain acetylcholinesterase activity

Order of Publishing in Issue: 
2
Volume :4
Issue :2
April, 2010 - June, 2010
Page No: 
648-659
Authors: 
R.P. Jaya Prasanthi [1] D. Chand Basha [2] and G. Rajarami Reddy [2]*
Address: 
[1] Department of Pharmacology, Physiology and Therapeutics, University of North Dakota School of Medicine and Health Sciences, Grand Forks, North Dakota, 58203, USA.
Address: 
[2] Division of Neurotoxicology, Department of Zoology, Sri Venkateswara University, Tirupati-517502, India.

Abstract : Alteration in the cholinergic system is one  of the major pathological hallmarks of lead (Pb) toxicity. Pb-exposure alters acetylcholinesterase (AChE) activity in brain regions which control learning and cognitive behavior. The present study is focused on the beneficial effects of calcium (Ca2+) and zinc (Zn2+) supplementation on Pb induced alterations in AChE activity in the brains of developing and adult mice. Mice were lactationally exposed to Pb (0.2% and 1%) and supplemented with either calcium (Ca2+) or zinc (Zn2+) and the synaptosomal AChE enzyme activity was analyzed in cortex, hippocampus, cerebellum and medulla of brains excised on postnatal day (PND) 14, 21, 28 and 3 months. The histochemical staining for AChE was also performed in the sections of mice brain. Exposure to Pb resulted in a significant decrease in the activity and staining intensity of AChE. Interestingly the supplementation with Ca2+ or Zn2+ reversed the Pb induced effects on AChE activity and expression. These findings strongly support that calcium and zinc supplementation significantly protects the brain from the Pb-induced alterations in cholinergic system.

Keywords: 
lead, calcium, zinc, acetylcholinesterase, synaptosomes
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