Production of Pharmaceutically Important Saponins from in vitro Regenerated plants of Chlorophytum borivilianum

Order of Publishing in Issue: 
9
Volume :6
Issue :4
October, 2012
Page No: 
458-463
Authors: 
Gayathri Bathoju and Archana Giri
Address: 
Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad- 85,

Abstract

Chlorophytum borivilianum belonging to the family Liliaceae is commonly called safed musli. It is a perennial rhizomatous herb widely distributed in the pan tropical regions which contains pharmaceutically important saponins. Among the saponins, stigmasterol and hecogenin as the major secondary metabolites are responsible for the various biological activities viz. aphrodisiac, antioxidant, anticancer and immune booster. Due to the immense medicinal uses the convemtional propagation is not sufficient to meet the commercial demand. So, micropropagation of such conventiaonally propagated plants is very essential to meet the commercial demand as well as to ensure easy storage and transportation of disease free stocks. The leaf sheath from in vitro raised plants was used for induction of callus on MS basal media fortified with 1mg/l of 2,4-D. Somatic embryogenesis and plant regeneration was observed from the callus obtained from MS basal media. Stigmasterol and Hecogenin were quantified from the callus and in vitro regenerated plants of C. borivilianum. Maximum stigmasterol production (3.265 mg/gm dry weight) was observed from plants regenerated from somatic embryos, which is 5.4 fold higher than the amount of stigmasterol in undifferentiated callus cultures (0.6 mg/gm). Maximum hecogenin production (43.55 mg/g) was observed in plants regenerated from somatic embryos, which is 27.9 fold higher than the amount of hecogenin in callus cultures (1.56 mg/gm). The present study reports higher production of medicinally important stigmasterol and hecogenin in regenerated plants in comparision to the callus cultures.

Keywords: 
Chlorophytum borivilianum (Safed musli), Stigmasterol, Hecogenin, Somatic embryogenesis, Quantification
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