Production and Purification of Alkaline Fibrinolytic Enzyme from Bacillus cereus GD55 under Solid State Fermentation and Screening for Industrial Applications

Order of Publishing in Issue: 
6
Volume :9
Issue :4
November, 2015
Page No: 
348-356
Authors: 
E. Venkatanagaraju[1*] and G. Divakar[2]
Address: 
[1]Department of Pharmaceutical Biotechnology and Microbiology, Acharya & BM Reddy College of Pharmacy, Soldevanahalli, Hesaraghatta, Bangalore-560107, Karnataka, India.
Address: 
[2]Department of Pharmaceutical Biotechnology and Microbiology,Acharya & BM Reddy College of Pharmacy,Soldevanahalli, Hesaraghatta, Bangalore-560107, Karnataka, India.

Enzymes are well known biocatalysts that execute a multitude of chemical reactions in the metabolism of almost all organisms. From an industrial perspective, only a restricted number of enzymes are commercially available and a few of them have found applications in large quantities. Microbial fibrinolytic enzymes are among the most important hydrolytic enzymes and have been studied extensively since the advent of enzyme technology. Alkaline fibrinolytic enzyme produced under solid state fermentation by Bacillus cereus GD55 was purified by ammonium sulphate fractionation (70%) and subjected to chromatographic methods. The purified enzyme produced 11.59 fold with recovery 6.8% by DEAE-Sephadex A-50 column chromatography and molecular weight was estimated to be 31 kDa by SDS-PAGE. This enzyme exhibited good compatibility with the commercial detergent Rin.The fibrinolytic potential of the enzyme was evidenced by the complete removal of blood stains from cotton cloth within 50 min of treatment. Bacillus cereus GD55 alkaline fibrinolytic enzyme with a diversity of activities, has great potential for application in a wide range of industry.

Keywords: 
Fibrinolytic enzymes, Bacillus cereus GD55, Solid state fermentation, Blood stains, Detergent compatibility.
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