Production and Characterization of Extracellular L-asparaginase from Estuarine Actinomycetes Species by Submerged Fermentation Process

Order of Publishing in Issue: 
Volume :7
Issue :1
January, 2013
Page No: 
Koteswara Rao Chinta [1], J. Srinivasa Rao [2]*, Pulipati King [3], P. Jawahar Babu [2], M. V. V. Chandana Lakshmi [1] and Ch. V. Ramachandra Murthy [3]
[1] Centre for Biotechnology, Department of Chemical Engineering, A. U. College of Engineering (A), Andhra University, Visakhapatnam-530003.
[2] Department of Biotechnology, Bapatla Engineering College, Bapatla-522 101
[3] Department of Chemical Engineering, A. U. College of Engineering (A), Andhra University, Visakhapatnam-530003.

Abstract : Actinomycetes were isolated from different estuarine sediments in the regions of Bay of Bengal off shores from Parawada to Visakhapatnam, India for extracellular Lasparaginase production. 5 different samples, 20 Actinomycetes strains were isolated. Among them 5 strains showing pink colour zones with high intensity were selected for the production of L-asparaginase. Out of 5 Actinomycetes, Strain PW-A12 shows maximum L-asparaginase activity with a yield of 3.933 IU/ml for 24 hrs Submerged Fermentation time. Further, optimizations of parameters were conducted with the strain PW-A12, by consecutive evaluation method. Maximum enzyme production with a yield of 5.73 IU/ml was observed with 4% peptone at pH 7.5. Reduced enzyme production was observed (5.6 IU/ml) while using on Yeast extract concentration of 1.2 gm /100 ml (w/v) and at a pH of 7.0 found to be optimal culture condition by the strain Streptomycetes species PW-A12 by submerged fermentation Process. Hence the present study indicated a scope for exploring estuarine Actinomycetes as a source for extracellular L-asparaginase production.

Estuarine Actinomycetes, estuarine sediments, L-asparaginase, Streptomycetes species.
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