Production of á-amylase from agricultural byproducts by Humicola lanuginosa in solid state fermentation

Order of Publishing in Issue: 
7
Volume :3
Issue :2
April, 2009
Page No: 
172-180
Authors: 
Ravi Kant Singh*a, Shashi Kumarb, and Surendra Kumarb
Address: 
aDepartment of Biotechnology, IMS Engineering College, Ghaziabad, U.P. India
Address: 
bChemical Engineering Department, Indian Institute of Technology Roorkee, Roorkee 247 667, Uttarakhand, India

Abstract:
 
In this study á-amylase activityexpression in Humicola lanuginosa wasevaluated under different environmentalconditions using solid state fermentation (SSF)on different agricultural byproducts. The solidsupports for á-amylase production in SSF processwere wheat bran, wheat straw, rye straw, andcorncob leaf. Wheat bran has been found to yieldmaximum production of á-amylase among thesesolid substrates. Effects of process variables,namely incubation time & temperature, initialmoisture content, pH, supplementary carbonsource, and inoculum level, on production of á -amylase have been studied, and accordinglyoptimum conditions have been determined. It hasbeen found that the á-amylase production is thehighest at 144 hr incubation period, 50ºCincubation temperature, 90% initial moisturecontents, pH of 6 and 20% inoculum level. Solublestarch has been found the best supplementarycarbon source.

Keywords: 
solid state fermentation, Humicola lanuginosa, agricultural byproducts, á–amylase, process optimization, solid support.
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