Abstract:
 
In this study á-amylase activityexpression in Humicola lanuginosa wasevaluated under different environmentalconditions using solid state fermentation (SSF)on different agricultural byproducts. The solidsupports for á-amylase production in SSF processwere wheat bran, wheat straw, rye straw, andcorncob leaf. Wheat bran has been found to yieldmaximum production of á-amylase among thesesolid substrates. Effects of process variables,namely incubation time & temperature, initialmoisture content, pH, supplementary carbonsource, and inoculum level, on production of á -amylase have been studied, and accordinglyoptimum conditions have been determined. It hasbeen found that the á-amylase production is thehighest at 144 hr incubation period, 50ºCincubation temperature, 90% initial moisturecontents, pH of 6 and 20% inoculum level. Solublestarch has been found the best supplementarycarbon source.