Plant growth promotion by bacterial inoculum with different conditions and estimation of enzyme activity from germinating seeds and bacterial isolates

Order of Publishing in Issue: 
6
Volume :12
Issue :1
January, 2018 - March, 2018
Page No: 
53-64
Authors: 
Navendra Uniyal#, Akansha Chauhan#, Komal Sharma and Debasis Mitra*
Address: 
Department of Biotechnology, Graphic Era University, 566/6 Bell Road, Clement Town, Dehradun 248002 Uttarakhand, India.

Plant health and other parameters are dependent on plant growth promoting (PGP) abilities which are further dependent on the microflora in the plant rhizospheric soil. In this study both the sterile and non-sterile soils of Dehradun were taken for the PGP and the seeds of Cicer arietinum were taken for the vigour index with the treatment of NKA1 (phosphate solubilizer). Our results showed that the VI of NssT† (1599.532) &NssT* (1630.729) were high as compared with SsT† (1069.866) &SsT* (606.554). When the dry weight of roots and shoots of Cicer arietinum were calculated there were no significant difference in these treatments. Other study was focused on estimation of â-amylase activity of germinating seeds and á-amylase activity of 26 bacterial isolates. NKA1 and isolates genomic DNA was isolated by the phenol-chloroform DNA extraction method and DNA has no traces of contaminants. Overall study showed that plants are favorable for the growing in natural soil with potential microbes. Rhizobacterial population mainly promote & helps the plant growth via different mechanism and microbial inoculants improve plant health and yield.

Keywords: 
PGP, Cicer arietinum L., Vigor index, Enzyme activity.
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