Plackett-Burman design for screening of fermentation process parameters and their effects on L-methionine production

Order of Publishing in Issue: 
8
Volume :14
Issue :2
April, 2020 - June, 2020
Page No: 
182-189
Authors: 
Venkata Narayana .A[1], Venkateswarulu T.C[1,*], Ranganadha Reddy .A[1], Ranga Rao .A[1], Abraham Peele.K[1], John Babu .D[1], Asha .S[1], Sumalatha .B[2], Sudhakar .P[3]
Address: 
[1,*]Department of Biotechnology, Vignan’s Foundation for Science Technology & Research, Vadlamudi-522213, Andhra Pradesh, India.
Address: 
[2]Department of Chemical Engineering, Vignan’s Foundation for Science Technology & Research, Vadlamudi-522213, Andhra Pradesh, India.
Address: 
[3]Department of Biotechnology, Acharya Nagarjuna University, Guntur-522210, Andhra Pradesh, India.

In the present study, economical fermentation medium process was developed to achieve the maximum production of L-methionine by Corynebacterium glutamicum through screening of different nutritional and physical parameters by Plackett-Burman design. A total of eleven process variables such as plantain as carbon source, groundnut as nitrogen source, CaCO3, K2HPO4, KH2PO4, biotin, MgSO4. 7H2O, inoculum size, agitation speed, volume ratio of medium/fermenter and pH were used for screening experiments. The PBD model results suggested that seven variables namely plantain as carbon source, groundnut as nitrogen source, CaCO3, MgSO4. 7H2O and KH2PO4 had shown a significant effect on L-methionine production while remaining six variables didn’t show a much effect on Lmethionine production. The R2 value (0.99) of analysis of variance (ANOVA) recommended that the model used for response prediction is significant (p<0.05). In comparison with the unoptimized medium, 24% higher L-methionine production was obtained from the optimized medium and L-methionine production was found to be 5.6 g/l.

Keywords: 
L-methionine, Corynebacterium glutamicum, Optimization, Plackett-Burman Design.
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