The phytochemical study of the seeds of Mucuna monosperma DC. revealed the presenceof flavonoids, sterols, triterpenoids. The separated compounds were identified based ontheir melting points, IR, 1H NMR and mass spectral data, such compounds were acacetin,luteolin, b-sitosterol, stigmasterol, ursolic acid and betulinic acid. The antimicrobial activity ofchloroform and methanolic extract of seed powder was investigated. The methanolic extractexhibited marked antibacterial activity against gram positive, gram negative organisms andfungi.