Phytochemical Analysis and Antimicrobial Potential of Abutilon indicum Stem Extracts against GUTI Pathogens

Order of Publishing in Issue: 
10
Volume :8
Issue :2
April, 2014
Page No: 
192-203
Authors: 
Ch. Suvarna Lakshmi[1]*, A. Uma[1]*, R.S Prakasham[2], L Jayalaxmi[3] and N. Chandrasekhar[1]
Address: 
[1]Centre for Innovative Research (CIR), IST, Jawaharlal Nehru Technological University Hyderabad (JNTUH), Kukatpally-500 085, Hyderabad, India
Address: 
[2]Biochemical and Engineering Sciences, Indian Institute of Chemical Technology (IICT), Hyderabad, India 3Department of Microbiology,Osmania Medical college, Hyderabad, India
Email-ID: 
vedavath1@jntuh.ac.in

Abutilon indicum is a well-known traditional medicinal shrub and used as an agent of anthelminthic, hepatoprotective and hypoglycemic. However limited studies are available on its potential against Genitourinary tract infections. The present investigation deals with the analysis of possible phytochemicals exist in the stem of Abutilon indicum followed by evaluating its antimicrobial potential against various pathogenic microbes involved in Genitourinary tract infections. The phytochemicals analysis showed the existence of flavonoids, steroids, tannins, phenols etc upon subjecting to sequential extraction process employing n-hexane, ethyl acetate, methanol and aqueous solvent systems. Evaluation of extracts against a panel of GUTI-pathogens revealed that against all the tested extracts, the ethyl acetate extract showed considerable inhibitory effect against all the pathogens. The extract was separated by TLC and silica gel column chromatography to fractionate the bioactive constituents. Evaluation of chemical nature of ethyl acetate fractions revealed that the presence of alkaloids, steroids, phenolic compounds, tannins, tripenoids and saponins. The isolated compounds were further screened for antimicrobial activity against GUTI. Bioactive alkaloid fraction showed 95.10% purity by HPTLC was subjected to FTIR analysis.

Keywords: 
Abutilon indicum; Ethyl acetate, Bioactive, GUTI.
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